Contemporary LGBTQ+ literature/writers in Ecuador?

I am looking to find contemporary (2005 and onward) LGBTQ+ literature/texts (novels, poems, digital literature, correspondence, social media writers) by Ecuadorian writers (preferably LGBTQ+ too,...

04 February 2020 9,780 3 View

What are the risk and resilience factors for student mental health?

Students in their final year of a Psychology degree at the Royal Holloway University London are researching factors affecting student mental health. All students studying in the UK please...

27 November 2019 241 2 View

Best experimental setup for ROS measurement after plant extract treatment using RAW 264.7 and H2DCFDA?

I am treating RAW 264.7 macrophages with some plant extracts dissolved in ethanol and measure if they decrease ROS/NO production. I have been having some trouble measuring ROS. A condensed...

20 August 2019 9,643 3 View

Why do no bands appear during glycoprotein staining?

Dear all, I'm trying to stain sugar groups attached to my protein with Glycoprotein staining (Thermo Scientific Pierce Glycoprotein Staining Kit #24562) after gel electrophoresis. I've added 4...

07 April 2019 3,224 2 View

How do I know that I can trust the chemical analysis results of surface and ground water quality samples from a subcontractor?

I was unable to perform the sampling and analysis for 40 surface water and 20 groundwater samples, so I hired an in-country consultant firm to do it. The results that they returned were difficult...

21 October 2018 8,937 10 View

Can anybody help me? Why is the genetic variance negative?

12 September 2018 1,959 14 View

Why can't we mate Thy-1 GFP to other transgenic lines?

We have a Thy-1 GFP mouse in our colony. We maintain the mouse as homozygous because the heterozygous animal did not show a strong enough signal. There doesn't appear to be any issues with the...

29 August 2018 3,576 4 View

What is the typical duration of a cell invasion assay? 24-48 hours, longer?

Can cell migration/invasion in the presence and absence of pharmacological inhibitors be tested for 1 week using this assay?

01 April 2018 5,885 5 View

Problems with protein purification by ion exchange chromatography

Hello everyone, I am trying to purify my protein (enzyme) expressed in Pichia pastoris through ion exchange chromatography. The protein is extracellular, so I extract the supernatant after...

27 July 2017 4,012 5 View

Ellipse on PCA analysis

Can I ask what the ellipse argument is used for in PCA? How to analysis the data by different ellipse shape and different direction in statistics? Is there any example for interpretation data by...

30 May 2017 7,219 1 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

No title

02 March 2021 3,060 3 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Suggestions for occupational stress surveys?

I am currently writing a masters thesis on reasons for occupational stress in the early childhood classroom, and want to include a short survey assessing participants work-related stress levels. I...

24 February 2021 8,678 2 View

How COVID-19 has affected mental health?

The pandemic has led to depression and anxiety and its negative effects shouldn't be ignored. However, it has also led some people to become more resilient! What do you think? Thank you.

21 February 2021 4,733 19 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View

Does qualitative research using closed and open ended survey and semi structured interviews qualify as triangulation?

I am using qualitative research to look at employee motivation. I will use a closed and open ended survey (for biodata information and general views to motivation) and semi structured interviews...

18 February 2021 436 11 View

Can medicinal plants really play an inherent rule in the COVID-19 tackle?

Numerous scientific reports cited plant extracts with potential antiviral activity and even anti-COVID-19? Why these findings are neglected? I remember very well the warning released by the WHO to...

17 February 2021 2,141 9 View

Should I include less important items in con-joint survey experiment?

I plan to do a conjoint survey experiment in which I randomly change attributes of hypothetical job candidates and show two types of potential candidates with different attributes. Then, I ask...

16 February 2021 5,812 1 View

How can I interpret a ratio variable?

Dear colleagues, I am running a regression in which I have the following regression FoodExp = 67.04 DepRatio +.....e My interest is in the interpretation of the coefficient on the dependency...

16 February 2021 2,058 2 View