Having trouble getting it to grow despite proper supplementation, but M.tuberculosis and M. intracellulare seem to do well in it.
I am writing an article for my blog regarding orchid viruses aimed at home gardeners. It will include details of home testing kits their use and accuracy. I also want to include details about...
26 June 2024 7,422 1 View
I am look at Job evaluation and it feels that the advantages of point-factor rating seems to also be the disadvantages - for example it is objective, but also subjective
14 May 2024 4,747 0 View
For my thesis, im doing research in finding the leadership style that is the best match with organization X to maintain work engagement. For this research I'm going to interview 10 employees and...
19 March 2024 539 0 View
Hi! I am graduating with my masters in Marriage & Family therapy the end of February & want to continue my education with a PhD. I applied to Florida State but that did not work out. What...
07 February 2024 3,341 2 View
Hello, I'm currently in need of performing Loop-Mediated Isothermal Amplification (LAMP). There are modifications in both the Bst DNA polymerases from NEB and ThermoFisher. Has anyone had...
31 January 2024 2,534 1 View
Image and text encryption
26 January 2024 1,223 4 View
I am looking for access to some publicly (or cheaply) available free-text clinical records, to help with the development of an AI tool. I am aware of CPRD (too expensive, not free text) and...
15 January 2024 7,535 1 View
Hello all. We are trying to get access to prostate cancer cell lines that were derived from patients who were Black or of African descent. There are numerous relevant cell lines reported in the...
07 December 2023 4,969 3 View
I mixed EDTA mouse plasma with standard gel sample buffer and got very smeary image on Western with no distinct bands. Is there something in the plasma that needs to be removed before I run gels?
06 December 2023 8,966 1 View
I've never seen anything other than bacteria and this doesn't look like anything I can find on Google! Is it fungus?? This is a plate of neurospheres if that helps.
29 November 2023 7,553 2 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View