Romit Seth

3 Questions 8 Answers 0 Followers

Questions related from Romit Seth

Can multiple assembly with different platforms be used simultaneously for analysis in RNA Seq experiment ?

we have performed assembly using both CLC work bench (average length: 674 bp; N50: 890 bp) and TRINITY (average length: 725 bp; N50: 928 bp) and performed the analysis from both of them...

17 April 2017 6,844 4 View

How can we design the most precise Real Time Primers from annotated transcripts/Contigs with querry coverage less than 70% in transcriptome data ?

Whether the ORF region to be considered for primer designing should be between start and stop codon or some other parameter should be used to encapsulate maximum gene spanning region in annotated...

03 July 2016 4,574 3 View

Why do I get multiple contigs with same annotation in transcriptome analysis ?

what could be the possible reason of getting multiple contigs with same annotation in transcriptome analysis. If we are getting no. of contigs showing same annotation then how to select the...

05 January 2016 7,891 4 View