Romit Seth

5 Questions 21 Answers 0 Followers

Questions related from Romit Seth

Can plant gene specific inhibitors be used to study plant physiological response to abiotic factors in non modal plants ?

I want to know whether can we use inhibitors for certain specific group of genes like HSPs, to study their role in abiotic factors apart from validating the gene on heterologous expression...

11 October 2020 7,658 6 View

Can multiple assembly with different platforms be used simultaneously for analysis in RNA Seq experiment ?

we have performed assembly using both CLC work bench (average length: 674 bp; N50: 890 bp) and TRINITY (average length: 725 bp; N50: 928 bp) and performed the analysis from both of them...

17 April 2017 6,949 4 View

Can we use edgeR to analyse DGE of 3 samples (2 in triplicates and 1 in duplicate conditions) ?

whether three samples with 2 in triplicates and one in duplicate can be used to analyse differential gene expression analysis using edgeR. what could be the better way, whether all sample in...

06 March 2017 9,015 3 View

How can we design the most precise Real Time Primers from annotated transcripts/Contigs with querry coverage less than 70% in transcriptome data ?

Whether the ORF region to be considered for primer designing should be between start and stop codon or some other parameter should be used to encapsulate maximum gene spanning region in annotated...

03 July 2016 4,656 3 View

Why do I get multiple contigs with same annotation in transcriptome analysis ?

what could be the possible reason of getting multiple contigs with same annotation in transcriptome analysis. If we are getting no. of contigs showing same annotation then how to select the...

05 January 2016 7,990 4 View