What are the factors responsible for non uniform decrease in corrosion rate?

I am working on the corrosive effect of food condiments juices on some selected metals using weight loss experiment. But while plotting the graph, I found out that the decrease in corrosion rate...

21 January 2021 6,688 4 View

Granger causality lags for comparing multiple simulations?

Hi all, I'm running neural network simulations and using granger causality to compare the Granger drive from one population to another. Generally speaking I'm using a code to find the Akaike...

16 November 2020 925 3 View

Creating randomization matrices (null models) with abundance data in R (Ulrich & Gotelli 2010)?

Hello, We are aiming to create a simple randomization algorithm in R program with abundance data. Specifically, we are aiming to create the IT-Algorithm (Ulrich & Gotelli 2010, Ecology),...

25 March 2020 5,873 3 View

Are there special considerations for Filipin stock solution storage and reuse?

My lab recently purchased both the Sigma (F9765) and Cayman (70440) Filipin reagents. These reagents were resuspended in DMSO in the hood in the dark and aliquoted to avoid freeze/thaw. Stock...

05 November 2019 8,392 1 View

Quantifying trend over time for bray curtis values?

Hello, I'm analyzing bray-curtis values over time. I would like to quantify if 1) there is a rapid or gradual change in bray curtis values, and 2) how long this change occurs. As a hypothetical...

19 February 2019 7,189 3 View

What causes this variable feature at the start of LiCoO2 charging voltage profiles?

I have made many LiCoO2 vs Li coin cells for charging and studying structual changes during the Li removal, but my voltage profiles do not follow the conventional behaviour in the literature. I...

09 September 2018 7,740 2 View

Setting Up LMM in SPSS: Comparing Two Groups (12 Individuals Each) Across 16 Time Periods?

Hello, My Project: I'm analyzing beetle species at carcasses predated on by GPS collared pumas in the Greater Yellowstone Ecosystem. I have 12 fresh carcasses that I've sampled each week. Each...

02 January 2018 9,524 3 View

After you create a mokken scale, can you get an overall score for the scale?

I'm exploring the concept that scales can be ordered and that certain items should carry more weight in a scale. I came across guttman scalograms and Mokken scaling techniques. Creating the...

22 November 2017 7,026 5 View

What causes false negatives in PCR reactions and how can I fix it?

I've recently been performing many pcr reactions and have only recently run into contradicting results. What once was coming out as positive is now negative. I run positive and negative controls....

05 April 2017 786 1 View

Best scale(s) for measuring job satisfaction, organizational commitment, turnover intentions and withdrawal behaviors?

08 January 2017 3,751 7 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

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03 March 2021 5,360 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View