Shada Elhayek

6 Questions 12 Answers 0 Followers

Questions related from Shada Elhayek

How can I remove bubbles form FACS Canto II ?

Dear all, I am having a technical problem with our FACS Canto II. It seemed somehow the device picked up some air bubbles and now it does not complete the fluidics start up neither the purge. I...

08 January 2023 4,492 4 View

Any recommendation for Lentiviral transduction of murine T cells?

I am trying to transduce mouse T cells with CAR constructs using lentivirus. The protocol I am using for now seems inconvienent that transduction is not working at all. So briefly, I isolate T...

15 October 2021 1,372 0 View

Subcutaneous tumors of KP cell line, Does anyone have an idea about growth rate?

Hello Everybody I am using KP mouse cell line to induce flank tumors in OT-I mice, but still can't get any palpable tumors although I have injected my mice two weeks ago. I used 10*104 and 5*104...

14 January 2019 4,870 0 View

Expansion of non-antigen specific mouse CD8+ T cells. How can I get enough number of expanded CD8+ T cells?

Hello everybody I am working on expanding and activating CD8 T cells from mouse spleens for my experimental work, but i have some troubles with the number of cells I got after 3 days culture. I am...

06 November 2018 694 8 View

Is there a specific assay for carbonic anhydrase III in rats? How can I asses the enzyme activity after treatment?

I am screening some compounds for possible inhibitory activity on carbonic anhydrase III using animal model. I have searched the PubMed but i could not find any method that asses the enzyme...

26 March 2016 1,823 3 View

How can I stimulate vascular endothelial growth factor 2 in vitro in Hela, MCF-7 and HCT116 cancer cell lines?

I have some compounds expected to be inhibitors of tyrosine kinases, and i want to test their inhibitory action in vitro by ELISA. i used the cell lysis buffer recommended by the kits and...

10 October 2015 5,159 4 View