Many articles describe the suspension cell growth is somewhat limited by concentration, that was observed in my experiment, doubling time of 1e5 cells/mL was longer than 5e5 cells/mL.
The growth is suppressed when reaching around 2e6 cells/mL, from this results I'm wondering, what if I start growing cell from a single cell, does it survive, and how will it affect the doubling time? Does someone experience with such experiment, and if is possible, do any precaution need to be take during cell culture?
- Badges
- Science method
More Mu-Shen Chang's questions See All
Similar questions and discussions