Can it cause problem if i collect rosemary leaves in winter(no snowy but cold(-3˚C)) for extraction?
when you collect your samples you can keep them in the freezer until you will do exraction.
As I know cold isn't a problem, because you have to work in ice to keep a low temperature for DNA extraction.
If you want to extract DNA, it is not a problem, DNA must be the same. But for RNA and protein, the conditions is important if you plant to compare your samples with another species/conditions.
My best wishes!
Hello Latifa @ZHOURI & @Taras Pasternak
first of all thanks for your kindly answers, but i don"t mean extraction of DNA,RNA or ... . I mean to extract all the substances it contains.
Thanks! If your aim is to study meatbolism, and make some quantification, condistions of plant growth must be standard.
Changes in conditions will led to chnage in substances.
with what extract you want to compare current one?
Even leaf collecetd at different day time (morning and evening) may have different metabolites.
My best wishes!
hello again Taras Pasternak
based on you answer i conclude that the time of leaf gathering is importent so i'm looking for the best harvest time(season and part of a day)
thank you very much
Added an answer
Exactly, I agree with dr Taras, meatabolism of plant depends on time of prelevment biotic anf abiotic conditions especiallty photosyntetic periode. So you have to work in conditions (time of prelevment) already used and published by an other author, or you can take your samples at differents time and choose the best one to achieve your goals...
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