For this test, the bacterial cultures were streaked on Pikovskaya’s
agar medium (HiMedia Laboratories, India) and supplied
with tricalcium phosphate in Petri plates. Plates were then
incubated at 28 + - 2 degrees C for 72–96 h. The formation of
clear halo zones encircling the bacterial colonies indicated
phosphate solubilization.
1. What would be the concentration and volume of tricalcium phosphate if added to 1L media bottle
2. What would be the concentration and volume of tricalcium phosphate if added to Petri dish?
Do we add before autoclaving the media or after autoclaving?
According to Pikovskaya R, Pikovskaya RI. Mobilization of phosphorus in soil in connection with the vital activity of some microbial species. Microbiol. 1948;17:362–70,
PKO liquid medium consist of NaCl 0.3 g, Glucose 10 g, KCl 0.3 g, Ca3(PO4)2 - 5 g, (NH4)2SO4 0.5 g, MgSO4· 7H2O 0.3 g, MnSO4 0.03 g, FeSO4· 7H2O 0.03 g, distilled water 1000 mL, pH 7.0–7.2, agar 15-20 g, autoclave (30 min).
add 0.05 mL of your 100 g/L TCP stock solution to a 1L media bottle
Meida Vol/plate: 20 mL
Tricalcium phosphate (TCP) should always be added to the media before autoclaving (Autoclaving after adding TCP helps prevent contamination and ensures even distribution).
- Badges
- Science topic
- Similar topics
- Geoscience
- Asia
- India