I require a mRNA marker for microlgia for qPCR analysis and was wondering if IFNg would be a suitable candidate. I am looking at microglia in quail and commerical gene sequences are hard to come by but I have one for IFNg. I undertsand IFNg activates microglia so would this be a viable candidate?#
Thanks
It's not the most standard marker (MHCII probably is), but it should be a pretty decent marker. Isn't there already a quail genome though? If there is, why can't you use different markers?
The quail genome is a draft and there isn't a complete sequence for it. I asked a company to design primers for me for microlgia specific genes, such as CX3CR1. After sending them quail cDNA, they couldn't validate the primers to the best of their ability as they had synthesised primers based on the chicken genome. There is a quail sequence for IFNy that I could go with. I understand your point with MHCII but I am looking for microglia specificity as I know MHCII is expressed by astrocytes in the brain. Thanks for the reply.
Dear David as it was told by other friends, your marker is not so appropriate, currently i'm working on EAE model, and i've searched about microglia, eventually i understood that CD1125, CD45, Cx3cr1, Iba1, CD68 would be best marker. i suggest you to use IHC technique for assessing microglia activity. the article link is attached for you. its can solve your problem. maybe
http://www.sciencedirect.com/science/article/pii/S2211124713002969
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