Greenhouse Simulation using COMSOL Multiphysics ?

Have anybody tried to simulate greenhouses using COMSOL Multiphysics? and is it doable?

26 December 2020 803 1 View

Did anyone used microflow imaging to measure the size distribution of Multivesicular liposome which are charcterized with size (>3um)??

I have prepared Multivesicular liposomes using the following procedures: Multivesicular liposome formulations for the sustained deliv... Multivesicular liposome (MVL) sustained delivery of a...

18 November 2020 3,189 2 View

CROs or collaborators for assay of non-ATP competitive kinase inhibitors?

We have compounds that showed activities against variety of kinases. However, since they have been designed to bind to the peptide site on kinases, I need to investigate this mechanism. Please...

15 November 2020 2,040 3 View

I followed a common procedure to synthesize Fe3O4 nanoparticles. The problem is that XRD pattern does not show any crystal structure. Could you help?

Besides, I have performed different characterization tecniques to my Fe3O4 nanoparticles and all are similar to what is reported in literature. Those characterization techniques include FTIR, SAED...

22 July 2020 8,549 12 View

Please recommend biological investigation of newly identified anticancer compounds that specifically inhibit Src kinase?

We aim to publish ta high impact journals (If 6+). Compounds showed incredible activities on cancer cell line (submicromolar on several types). I need your recommendation on how to determine...

24 May 2020 1,230 3 View

To what extent do top-down language policies influence language use?

I'm often very skeptical about the language decisions and policies issued by governments or self-proclaimed language authorities to control the way people use language. Nevertheless, I'm just...

28 February 2020 5,904 4 View

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19 September 2019 5,877 1 View

Will a power fail affect my cDNA synthesis?

14 September 2019 8,969 3 View

What are the main reasons that may lead to failure of plans in tourism? and how to change it?

I believe that the concept of tourism planning is to face the expected operational errors, (on paper), before starting the implementation phase of the tourism project itself. Thus, this may lead...

08 June 2019 389 5 View

Which nucleases can degrade G-quadruplex ?

I am interested in the effect of different nucleases on G-quadruplex DNA. I see examples in the literature where exonucleases or endonucleases release G-rich sequence which consequently form...

31 October 2018 908 2 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Which transition metal salt precursor is best for doping? Why?

I would like to synthesize a transition metal doped TiO2 via solgel method. Hence, I would like to know which metal salt precursor to choose among Chloride, Nitrate, and Sulphate. A clear...

27 February 2021 4,285 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View