Probably.

I imagine that the LC-MS is reverse phase so you would need your sample to be in a solvent that is water miscible , which given these are metabolic profiles of cells lines samples this is likely the case.

Also, to prevent overloading the LC-MS column the concentration of the sample should be between 0.1 to 1 mg/mL. It's possible to go more concentrated without overloading the column, but that is depended on the column size and other factors, and I would err on the side of going less concentrated for the instruments long term function. You can always make a larger injection volume if it is too dilute.

So if the NMR sample's solvent is in a water miscible solvent and is not too concentrated, it should work well on an LC-MS.

If you want to recover the sample from NMR, probably you have to concentrate the sample (e.g. by speed vac, freeze-drying...) to make sure it is able to give you good results. It has no problem with the non-polar phase. For the aqueous phase, however, you will use a buffer with salts (in D2O) to maintain the pH so that it may cause trouble when you concentrate the cell lysate. Therefore, it will be better to separate at first. For example, 90% for NMR and 10% for LC-MS.

Although NMR has poor detection sensitivity, it can obtain information on the three-dimensional structure and dynamics of a compound at the same time. If you want to keep track of changes in metabolism over time, I think you should also use NMR.

Dear Saman,

In what solvent do you do your cellular extract (deuterated MeOD/D2O)?

Presence of exchangeable OD's will complicate the MS analytics substantially, because any exchangeable OH will be present as a OH/OD mixture...MS experts are normally not happy with this situation.

If you do the extract with MeOH/H2O I would split the sample prior of adding D2O lock. Normally a small aliquot of a NMR sample is adequate for LC-MS anyways.

I hope this helps

Alfred