What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View

Is Handheld XRF analyzer useable? and accurate in geochemical test?

What is the different between Lab XRF and Hanheld XRF, is it accurate and useable to analyze sedimentary rocks?

23 January 2021 9,941 6 View

Do you know any well-known kinetic model for agglomeration of gas hydrates or colloidal particles?

I have been trying to derive a kinetic model for aggregation of asphaltene molecules in molecular scale. I am looking for similar models in gas hydrates or colloidal particles in molecular scales...

21 December 2020 4,233 3 View

Does oil interfere Total Dissolved Solid determination in water?

Dear friends, this is regarding Total Dissolved Solids test. when we have a oil mixed sample how do we analyze TDS? when sample is filtered through a glass micro fiber filter paper, oil in the...

04 November 2020 5,065 3 View

UV-Vis Spectroscopy of Carbon Nano Tube ?

Dear all, I just started the UV-Vis Spectroscopy of Carbon nanotube (CNT). I just got confused to interpret the results. Would you please help me with these questions: 1- Why I cannot find the...

30 June 2020 4,676 7 View

Why do I not see any bands on SDS-PAGE while the Bradford colorimetric assay gives blue color ?

I am purifying a recombinant protein (MW 37 kD) using IMAC. I used the Bradford colorimetric assay (by adding a drop of the solution from the column to Coomassie blue G-250) on the wash and...

07 April 2020 3,068 9 View

How can connected between microbiology with histology or pathology?

any idea a bout how bacterial infection due to tissues pathology this could be a link between them or not.

08 February 2020 6,840 4 View

In meta analysis, what do you have to do with a research in which the dependent variable is broken into two variables ( y1 and y2)?

If you want to use the meta-analysis as your research method to investigate the relationship between two variables (ie x as independent and y as dependent variable), what do you have to do with a...

05 December 2019 5,666 3 View

Is there a way to test arsenic content in LPG streams up to ppb??

I working on adsorption of Arsine and phosphine from hydrocarbon streams such as propane, propylene, etc. Is there a test method that can be detect Arsine in the range of 1 ppb from these feed...

24 May 2019 5,007 1 View

Conductivity value not matching with other parameters?

10 March 2019 2,401 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View