Hi,

I do not know whether you are talking about human or animal osseus tissue... but, yes, you can.

http://www.ddk.com/PDFs/cryosectioningguide.pdf

https://books.google.bg/books?id=Dhn2KispfdQC&pg=PA350&lpg=PA350&dq=frozen+undecalcified+bone+tissue+sectioning&source=bl&ots=JAoFbyPBC6&sig=ACfU3U2ccgnRfggC_Sl-rVxJvy2FbPbn4w&hl=en&sa=X&ved=2ahUKEwjsndaGkYrkAhUKmIsKHYT-Aog4ChDoATAHegQICRAB#v=onepage&q=frozen%20undecalcified%20bone%20tissue%20sectioning&f=false (scroll up for additional information)

https://www.jstage.jst.go.jp/article/jhtb/26/1/26_61/_pdf

Tackling some possible issues:

Article Preparation of Thin Frozen Sections from Nonfixed and Undeca...

Article The use of commercially available adhesive tapes to preserve...

Also, have a look at these bone histology protocols:

http://www.boneandcancer.org/MOLab%20protocols%20since%2011-2005/F24%20Bone%20Histology%20Protocols%20from%20David%20Rowe%20Lab.htm

I hope this helps :)

I thank you very much Dr. Med. Valeria Tananska. I'm talking about animal bone tissue

You have to consider the status of bone if it is thin and fragile

Decalcified or not

How much thick sections you want to see

Following

I thank you @Satish Narula ....After the rats sacrification I just collected the bones and freezed them ... actually I want to make a histological section (photomicrograph) to evaluate the cell morphology before and after the treatment.

What type of treatment you are giving and it's effects on physical properties of bone have to be taken into consideration

You have to consider the size of bone sample for processing if inadequate you can not succeed