Can i use taqman gene expressn assay to detect a house keeping gene then use another taqman assay with different dye for the same gene rest of samp ?

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Is beta actin Rna stable?

in RTqPCR. if i have my target gene expression at ct 33 and beta actin at ct 17..i repeated and that's always the ratio..could my rna be degraded but i have good ct for beta actin how?

08 September 2019 6,957 4 View

Is there a difference between Rna expressed from cell culture and that extracted frim tissues?

if i can able to detect the transcript cd36 from cells but not from brain tissue .'.expected to have it'. ciuld it have more secondary conformational chnges than from cells?..just IDK

08 September 2019 5,637 5 View

Regarding Morris water maze test, if mice showed thigmotaxis in most of hidden platform training days whether reveciving LPS r control?

Should i exclude them from analysis? does LPS iP injection increases Thigmotaxis behavior?

02 March 2019 7,230 3 View

Nuclear protein PGC 1 alpha ? best way to extract from mouse brain ?

i have been struggeling to xtract it by ripa buffer ..then itake supernatent and heat in1 :1 ratio with 2x sample buffer at 95 degrees for 5 mins ..do yu have any suggestions ? it' s large ptn.so...

11 December 2018 1,983 2 View

If i add protein extract to sample buffer is there difference if i heated till 60 for 10 mins than at 95 for 5 mins?

10 mins at 60 degrees vs 5 min at 95 degrees

10 November 2018 714 7 View

Tips for large number of samples homogenization for rna extraction?

iam extracting RNA from brain tissues that are stored in -80 .should i get them all at same time from -80 which starting to homogenize some of them on ice or get few sames out of the -80 everynow...

10 November 2018 4,157 1 View

Is it better to reverse transcripe 1or 2ug of sample from brain tissue?

brain tissue reverse transcription..

10 November 2018 2,274 0 View

I cant get protein signals using RIPA buffer for extraction of one brain hemisphere ? (myhous keeping ptn is cytoplasmic ptn beta actin ? no signal?

i doubt i have a problem in my extraction procedures .. i homogenize my brain in RIPA buffer NACl 150 mM , 25mM tris , SDS 0.1%, Sdium deoxycholate 1%, Triton x 1%, edta 5mM ... i use it in 1ul...

10 November 2018 5,897 6 View

Can i multiplex 2 genes and singplex the rest in same run and compare results?

i mean i will do qpcr i will multiplex my housekeeping taqman gene (pl) vic dye with another taqman gene and will do the rest in singleplex format ? can i compare other genes expression to...

10 November 2018 2,868 3 View

Does alzheimer's disease affect novality seeking behavior in mice? or it's all about memory retrieval?

modified Y maze , would be glad if you posted advices /papers

09 October 2018 7,257 0 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View