Can i use same primers of a carboxylesterase gene in Locusta migratoria for that same gene in Zonocerus variegatus. Will the PCR work well?

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Giorgia Pertile

If the primer is not draw for the specific gene in Locusta, I think yes.

Navnit Kumar Ramamoorthy

Dear Temitope,

Related in what sense? Are you sure they have the same genes in them coding the enzyme? Have you done background study of the same? Get the specific gene sequences from databases and do a nucleotide match. If you happen to find similarity, then proceed with the PCR using the same primers.

Miguel Fernández-Niño

Hi. I don't think so. It seems that there is a lot of variation between nucleotides and aminoacids sequences in these genes. Check this paper: Opossum carboxylesterases: Sequences, phylogeny and evidence for CES gene duplication events predating the marsupial-eutherian common ancestor

January 2008BMC Evolutionary Biology 8(1):54

DOI: 10.1186/1471-2148-8-54

Hisham Abu-Ali

I agree with Miguel