Sterile-surface roots were macerated. From this material I isolated dozens of endophytic bacteria. Now I want to take this material to isolate endophytic fungi. I plan to use PDA medium with the only antibiotic that I have, ampicillin. Would it be okay supplement the PDA with ampicillin for this purpose?
I would suggest to use 2-3 different antibiotics including ampicillin for better results. Even with ampicillin you will get good results
Regards
Dr Rafiq Lone
I agree with Dr. Lone and i suggest to use better Chloramphenicol or Streptomycine. They gave me good result in the isolation of endophytic fungi for banana roots.
See Waller et al. (2001)-PLANT PATHOLOGIST POCKETBOOK, chapter 38 "micological media and methods" for most common antibiotics used in fungal media and its concentrations.
Regards
Einar
Thank you very much to all. Next week I will tell you how it works.
Go for it! It will work! Would be nice to share results with us!
Well, here is a preliminary result. Some fungi colonies were obtained, however some bacterial colonies appeared also. I'm happy with the result anyway. Thanks to all.
https://instagram.com/p/1J5EaaAaJq/?taken-by=morontafelix
How long did you incubate the culture? seem like the contamination is very dominant. Mind to share your recipe of preparation of ampicillin?
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