7 Questions 12 Answers 0 Followers
Questions related from Felix Moronta-Barrios
Dear RG community, I’d like to hear what do you think on the following observation: I have two bacterial strains, A and B. They don’t show antagonism between each other, they seem to be good...
06 September 2017 6,542 4 View
Using independent-culture approach, I have detected abundant 16S sequences belonging to Caulobacter sp. from agricultural soil samples, but I couldn't be able for isolating it using media like TSA...
03 August 2017 6,197 3 View
With the aim to analysing bacterial microbiomes in a cheaper way, I would like to tag the amplicons of each sample with primers barcodes. But I'm pretty new in this approach! Anybody here with...
31 May 2016 8,702 3 View
Could you recommend me any plate assay to achieve that? I aimed to check mutual antibiosis activities between different bacterial endophyte strains.
16 March 2016 479 1 View
How can I do a search for this kind of information? I can not menage the NCBI-Genomes as well. I need to construct a list with this information. Could you help me?
26 April 2015 9,410 1 View
Sterile-surface roots were macerated. From this material I isolated dozens of endophytic bacteria. Now I want to take this material to isolate endophytic fungi. I plan to use PDA medium with the...
25 March 2015 8,590 11 View
I isolated total DNA from rhizospheric soils of root rice. I amplified and purified 16S genes with Platinum Taq and universal primers. I just want to know if somebody has already worked with this...
17 March 2015 1,438 2 View