Felix Moronta-Barrios

5 Questions 9 Answers 0 Followers

Questions related from Felix Moronta-Barrios

Isolation of Caulobacter from environmental samples. How?

Using independent-culture approach, I have detected abundant 16S sequences belonging to Caulobacter sp. from agricultural soil samples, but I couldn't be able for isolating it using media like TSA...

03 August 2017 6,139 3 View

Has someone ever used DNA barcode for multiplex sequencing of bacterial microbiome?

With the aim to analysing bacterial microbiomes in a cheaper way, I would like to tag the amplicons of each sample with primers barcodes. But I'm pretty new in this approach! Anybody here with...

31 May 2016 8,672 3 View

How can I know all the rice endophitic bacteria sequenced to date?

How can I do a search for this kind of information? I can not menage the NCBI-Genomes as well. I need to construct a list with this information. Could you help me?

26 April 2015 9,362 1 View

Can I use PDA medium with ampicillin to isolate endophytic fungi from macerated roots?

Sterile-surface roots were macerated. From this material I isolated dozens of endophytic bacteria. Now I want to take this material to isolate endophytic fungi. I plan to use PDA medium with the...

25 March 2015 8,548 11 View

TOPO or pGEM vector for rhizophere 16S library?

I isolated total DNA from rhizospheric soils of root rice. I amplified and purified 16S genes with Platinum Taq and universal primers. I just want to know if somebody has already worked with this...

17 March 2015 1,406 2 View