What is the difference between using phosphate buffered saline with tween 20 vs tris buffered saline with tween 20? I ask this for the application of performing a western blot as well as stripping a western blot membrane to reprobe. I usually use PBST for developing my membranes, but now wish to strip a PVDF membrane and people recommended TBST.
Thank you.
Hi David
I am completely agree with Elizabeth E LeClair .
We have been using PBST for development as well as for stripping of membrane without facing any problems.
Good luck!
Our lab also normally tends to use the buffer recommended by the supplier's instructions and as much as PBST and TBST do not differ that much, we find TBST give a better performance in all applications during Western Blot, but I am sure that this too is relative.
Hello David,
Actually it depends on your protein of interest. There is another thing that some antibodies like PBST, some TBST. TBS is used instead of PBS when working with phospho-specific antibodies (sometimes phosphates cause problems by interfering with anti-phosphate antibody binding to your potentially phosphorylated protein so that using PBS can result high background with these antibodies) or when working with alkaline phosphatase (PBS interferes with alkaline phosphatase also).
Hi David:
TBS has a stronger ionic force compared to PBS. In some cases, when you use bad antibodies, buffer composition makes the difference in western blot aplications. When your antibodie is really good, buffer doesn´t matter.
Good luck!!!
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