Sayad Md Didarul Alam

5 Questions 8 Answers 0 Followers

Questions related from Sayad Md Didarul Alam

How to improve the yields of plasmid extraction?

My palsmid is around 9000 bp in pSectag2-MycHis-IRES-EGFP vector with ampicillin resistance and use DH-alpha E.coli.. It is highcopy plasmid. I tried both large and small volume of culture medium....

10 October 2018 2,618 5 View

I am trying to transfect S2 cell by pMT-Bip-GFP vector several time using cellfectine reagent. but did not succeed. Can anyone help me?

I am trying to transfect S2 cell by pMT-Bip-GFP vector. I tried several time using cellfectine reagent and also use Mirus Trans-IT reagents. but did not succeed. Can anyone help me?

10 October 2017 5,215 3 View

Could anyone please suggest me where I can get Tet induction system (plasmid) in cheap?

Could anyone please suggest me where I can get Tet induction system (plasmid) in cheap? The plasmid should not have any GFP gene.

10 October 2016 7,094 1 View

Could anyone please give me information about the detection of fluorescence signal from lateral flow immunoassay ?

Actually I am trying to develop a new LFIA method and want to use fluoresccence to detect the analyte.

06 June 2016 9,270 2 View

Could anyone please give me information about using of mobile phone for the detection of fluorescence signal from lateral flow immunoassay?

I would like to develop LFIA where I am trying to use mobile phone camera or any android software as a detector.

06 June 2016 249 0 View