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02 March 2021 932 1 View

Glass fiber content in a gypsum board ?

Any idea on how to get the volumic ratio of glass fiber in a gypsum board ? Thank you for your help !

01 March 2021 2,501 3 View

Tooth extraction and subsequent dental implant placement in Sprague-Dawley rats induce differential changes in anterior digastric myofibre size and ?

01 March 2021 7,254 3 View

Solution of 2D convection diffusion heat equation using MATLAB ?

This is a polymer heating problem. Polymer enters at around 180C (x=0) in the barrel. Also barrel initial temperature is 180 (t=0). Some heat Is added along whole length of barrel q. We have to...

15 February 2021 3,887 1 View

How is Initial GDP measured ?

Dear All, I am working on estimating Debt-Growth Threshold but confused about some points. My questions are; - How do we calculate initial GDP Per Capita? Shall we do it in STATA or excel file?...

15 February 2021 2,677 2 View

How to conduct mesh sensitivity/convergence analysis in MSC Marc Mentat?

Currently using Msc Marc Mentat for FEA. Now i conduct the mesh convergence/sensitivity analysis manually. So I need to know if there any automatic way to conduct the mesh convergence/sensitivity...

04 February 2021 3,310 3 View

What is the magnetic anisotropy constant for Permalloy (NiFe-80-20)? Can anyone suggest some papers?

Need to know the equation of anisotropy energy for permalloy and also the constants. Any reference paper will be helpful. Thanks for your time and cooperation.

26 January 2021 7,842 3 View

Similarity between these two waveform? Similarity in terms of Shape?

Hi, These are the outputs generated by a system. Green-colored (nr) is desired output, while the system generates Red-colored (nf). I also have a dataset in CSV file. For this waveform, What...

21 January 2021 929 2 View

What are the future research avenues in context of Urban Street, Outdoor thermal comfort and Neural Networks?

Dear academic community, I am a young researcher from architectural background. I am looking for possible novel approaches to evaluate outdoor thermal comfort in urban streets using artificial...

20 January 2021 7,964 3 View

Can we control the position and vibration, simultaneously, of a flexible link with a tip mass using only the actuator's input?

This is a boundary control problem of a hybrid ODE-PDE system. The system consists of an actuator, a flexible link fixed to the actuator, and a tip mass at the free end of the link. In most...

13 January 2021 8,050 2 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can I perform two way ANOVA in case of some missing groups?

I had control cells and performer ELISA analyses group (time 0). I stimulated cells with substance A and B and cultivated for 1, 2 and 3 hours. On each cells I performed ELISA. I have results...

17 February 2021 8,933 4 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

How do I change g/l into mM for bacto agar. I am trying to get the millimolar content of sodium and chloride percentage in bacto agar. How do I do it?

I am doing a salt tolerance-related experiment and for that I need to relate my salt levels to that in bacto agar. I don't want my salt concentrations to increase due to already salt content...

09 February 2021 4,158 1 View

What is the relationship that determines the depth of the mechanical cultivator with the age or size of the weed For crops planted in lines?

the great depth of the cultivator may lead to passage under the roots without killing them and may give negative results to break up the soil below the roots, increasing their activity, especially...

03 February 2021 3,198 7 View

Transformation into the Stbl3 chemo competent cells?

03 February 2021 1,111 5 View

How to Remove DNA From a Sample Without Rupturing Yeast?

I transformed a plasmid into yeast and selected for transformants by growing them in a selective media (liquid, not agar plate). My experiment requires that I continually grow/ split these...

01 February 2021 9,936 2 View

What is the composition of the solid and broth agar to grow Streptomyces from freezed sample? what are extraction steps of products from broth agar?

Please what are the composition of the solid agar could I use to grow the bacteria? what are the composition of the broth agar could I use to grow the bacteria and how to extract the natural...

26 January 2021 5,006 1 View