My undergrad thesis is all about contamination of water buffalo oocytes in the in vitro culture. Now, i've been researching for a possible antimycotic which I can use as a treatment. However, I've found Amphotericin B but it's too expensive, now I'm considering Ketoconazole as an alternative.
Does anyone tried using this? And may I know the concentration of it? Or could you suggest any other antimycotic which are not expensive?
Unless you find multiple studies that made use of ketoconazole as antifungal for cell culture treatments, I advise you not to use it. Ketoconazole has been documented to antagonize GRs (glucocorticoid receptors) and if your aim is to maintain the integrity of the cells after an antifungal treatment, your drug of choice might not be appropriate.
Check if this supplement might be of lower price that Amphotericin: http://www.invivogen.com/fungin
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