This is my undergraduate thesis as it will tackle the contaminants in the in vitro culture of oocytes. Furthermore, my professors recommended me to use Molecular Biology to identify these fungal contaminants and prevent them when I have already identified them. As for the prevention, what is the best measure you can advice to me? Can I use fungicide or just stick with the sterility of the laboratory?
Thank you!
Gamal Enan
You have to work under completely asceptic conditions and in Laminar Flow Cabinet containing filtered air. Sterilize your hands and sterilize the cabinet you work in .Also lab. atmosphere should be sterilized overnight with UV and switch off in the morning befor you enter to your Lab. Also you can use fungicides
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