Hello,
I have HisG tag monoclonal primary antibody in my lab that are purposed for western plotting analysis of N-terminal tags with 6x His-Gly tags. Can this antibody be used with C-terminal his-tags that don't contain glycine?
Hi Mostafa Zaher ,
I'm not a Western-Blot expert, but I think: Yes, of cause. It should not make a difference where in the fusion-protein (N or C-terminal) the detectable anti-gen is.
Best wishes
Soenke
Hello,
It should work because the antibody is raised against 6X histidine stretch. It will be able to detect multi-histidine patches on different sites.
Yes, you can use a HisG tag monoclonal antibody with a protein that has a C-terminal 6-His tag. The key to successfully using a His tag antibody is to ensure that the antibody recognizes the histidine residues within the His tag, which is typically composed of 6 consecutive histidine residues.
The specificity of the antibody for the His tag sequence is more important than whether the tag is located at the N-terminus, C-terminus, or elsewhere in the protein. The histidine residues in the tag are what the antibody is designed to recognize, and they are typically exposed on the surface of the protein.
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