Mostafa Zaher

4 Questions 1 Answers 0 Followers

Questions related from Mostafa Zaher

Strategies for Forming a Functional Protein Complex from Separately Expressed Multiepitope Proteins in E. coli ?

I am working on expressing two separate proteins (domains) in E. coli to avoid the challenges associated with expressing a single large protein (83 Kda). These proteins do not naturally form a...

24 March 2025 2,577 1 View

Can I use HisG tag monoclonal antibody with C-terminal 6-His tag?

Hello, I have HisG tag monoclonal primary antibody in my lab that are purposed for western plotting analysis of N-terminal tags with 6x His-Gly tags. Can this antibody be used with C-terminal...

24 October 2023 5,033 3 View

Can Ammonium acetate be used in salt solution of E-coli-based in-vitro translation system instead of ammonium glutamate ?

Hello, I am currently constructing E-coli based in vitro translation system that usually utilize ammonium glutamate in reaction salt solution according to recent publications and published...

08 March 2021 9,627 0 View

Do ammonium glutamate considered as necessity in salt sloution for Ecoli-based Cell free protein synthesis platform ?

Hello, I am constructing an E-coli based Cell free protein system (CFPS) reagent, but I can't find ammonium glutamate from the local suppliers or from MP biochemical local disruptors. Can I...

14 August 2020 3,994 2 View