Can I use glycerol from vegetable source(vegan) for e.coli banking and freezing?It is USP grade but from veg source
Yes, there is no problem in using it. The source doesn't make a difference, it is the same chemical.
Kindly check https://www.addgene.org/protocols/create-glycerol-stock/
Follow the steps for Inoculating an Overnight Liquid Culture. After you have bacterial growth, add 500 μL of the overnight culture to 500 μL of 50% glycerol in a 2 mL screw top tube or cryovial and gently mix. Note: Make the 50% glycerol solution by diluting 100% glycerol in dH20. https://www.addgene.org/protocols/create-glycerol-stock/
Glycerol as a cryoprotectant depresses the freezing point of bacterial cells, enhancing supercooling. It does so by forming strong hydrogen bonds with water molecules, competing with water-water hydrogen bonding. This disrupts the crystal lattice formation of ice unless the temperature is significantly lowered. http://2011.igem.org/Team:Cambridge/Protocols/Glycerol_Stocks
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