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My culture medium consists of elements mentioned below, but still the OD doesn't exceed 0.3-0.5!!(on LB the OD reaches 5!)what am I missing? Ammonium chloride Ammonium sulfate...
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I can see with bare eyes that my defined medium for bacterial culture has a pretty high opacity, but the OD doesn't exceed 2 at 600nm.I checked the sterility of my glassware and medium, the medium...
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Can I use glycerol from vegetable source(vegan) for e.coli banking and freezing?It is USP grade but from veg source
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