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I have invitrogen Top10 competent cells that do not grow on defined medium containing leucine...What do you think the problem can be?Is another aminoacid essential? Genotype: F-mcrA...
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I want to make blood agar using my own blood sample. How should I do that?What do I have to do after taking my blood sample so it could be used in the culture medium?
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I used a defined medium to grow my DH5alpha bacteria.It grows pretty great finally, but the lag phase is tooooo long(about 30 hours!)and afterwards it starts to grow fast and it reached a high...
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My culture medium consists of elements mentioned below, but still the OD doesn't exceed 0.3-0.5!!(on LB the OD reaches 5!)what am I missing? Ammonium chloride Ammonium sulfate...
22 December 2020 700 5 View
I can see with bare eyes that my defined medium for bacterial culture has a pretty high opacity, but the OD doesn't exceed 2 at 600nm.I checked the sterility of my glassware and medium, the medium...
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Can I use glycerol from vegetable source(vegan) for e.coli banking and freezing?It is USP grade but from veg source
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