How to export ZEN 3D time series?

I want to export 3D time series movie from ZEN. I found that I can only export one 3D image with its X or Y rotation instead of 3D-time series? I tried to use Fiji with the function of Z...

07 February 2021 5,365 1 View

HPLC separation for methyl salicylate, camphor, thymol and menthol?

I have been recently trying to develop a method to separate the above compounds. Most methods tried were adapted from literature using RP C8 or C18 column with ACN/MeOH and buffered water at pH 2...

28 December 2020 4,426 6 View

How to extract the different components at a specific frequency between two spectra?

Dear everyone: Hello! Sorry that my question may take up your valuable time. Recently I have a technical problem in the data processing. We have conducted a series of wind tunnel tests in order...

18 December 2020 8,847 2 View

Has anyone extracted plasmid DNA from the Dmp1-GFPtopaz plasmid?

I just bought a plasmid from addgene, the name is DMP1-GFPtopaz. However, I could not extract the vector DNA by using Qiagen DNA purification kits from the bacteria. the bacteria growth was very...

05 November 2020 2,458 3 View

May I know is Triple Negative cell line MDA-MB-468 is auto fluorescence ?

I am doing immunofluorescence using MDA-MB-468 cell to determine ICAM-1 and E-cadherin with Santa Cruz Biotechnology ICAM-1 (G-5) mouse anti-human (Primary antibody), Santa Cruz Biotechnology...

27 August 2020 1,641 4 View

Can we use pig imune serum to perform a passive immunization in mice?

We have vaccinated pig with antigens and then, we would like to know whether we can use mice to perform a passive protection assay by using the pig immune sera. Do you know pig macrophages can...

15 April 2020 2,510 2 View

How to statistically compare two different measurements for the same factor in a logit regression?

My key independent variable in my regression (logit) is the product's degree of spanning. I have two different measurement methods for this factor. And how can I statistically compare?

10 April 2020 3,679 3 View

Where is freestream velocity used in the noise prediction of helicopter rotor in forward flight condition with an observer fixed relative to rotor?

I am not so clear the use of freestream velocity in FWH equation when helicopter rotor was in forward flight condition and observer position was fixed relative to the rotor. The description of...

21 March 2020 8,631 3 View

Can you lyophilize micelles with hydration media such as PBS and HBS?

Many thin-film micelle formulation protocols use PBS and HBS as hydration media and involve a lyophilization step. When they use the products for in vivo treatment, they redissolve the lyophilized...

27 January 2020 4,964 2 View

Should drug encapsulated micelles solution clear out after PBS hydration?

I have been using the thin-film method to encapsulate drug with DSPE-PEG5K. After obtaining the thin film, I added 2ml PBS for hydration. After a short sonication to help dissolve the thin film,...

15 January 2020 6,502 3 View

Is there a way to get rid of TEA in a TLC plate in order to do proper staining?

Dear colleagues: I have been trying to establish a few HP-TLC methods in the lab. In general, for quantification purposes, I prefer CuSO4+acid charring, to primuline staining. Lately I have been...

20 January 2021 9,019 1 View

What effect will different solvents have on the ATRP reaction?

I want to do an ATRP reaction, synthesize an ATRP initiator, use DMF as a solvent, and mix DMF, BIBB and TEA to turn red. Why does it turn red? Xylene has been used as a solvent before, and there...

04 January 2021 8,034 3 View

Dear professors, i am confused with how to figure out the tea bag index?

Hello, i am confused with how to figure out the tea bag index,could you please tell me how to compute the letter "a" and "k". Can i use R or Sigmaplot to fix it?

09 December 2020 6,705 3 View

Can you please complete an onlinse survey? (only if you are 18 or over and you consume herbal tea or tisans)?

Hello, I am a research student in MSc Food Science and Innovation at Manchester Metropolitan University for 2019-2020. I would hereby like to invite you to take part in my dissertation research...

30 November 2020 528 2 View

What does this ghost peak come from?

We analyse oligonucletide using Ion-pair reversed phase chromatography. The mobile phase A is composed of triethylamine (TEA), hexafluoroisopropanol (HFIP), water. And the mobile phase B is the...

27 September 2020 4,210 3 View

How can I remove triethylamine tfa salt from my peptide?

I'm now purifying a modified tripeptide which is quiet non-polar and sensitive to acidic condition. Thus I added triethylamine (TEA) to neutralize the HPLC fraction which contains 0.1% TFA and...

07 August 2020 5,396 3 View

How to measure tea powder sample into ppm unit (not the extract, just the sample)?

If I soak 1 g tea powder into 100 mL aquades for 12 / 24 hour, can we assume that it is a 10000 ppm sample, not the extract, just the tea powder liquid (sample)? and if Yes, could you give me...

13 July 2020 1,693 7 View

Is taking coffee and tea without milk beneficial for our health?

Consumption of tea and coffee without milk is considered beneficial for our health. Can you please explain the reasons behind it? what can be other methods for improving sensory characteristics...

14 May 2020 6,096 23 View

Is comparing some rates among the patients with respective rates in general population appropriate in epidemiology?

Is it appropriate to compare rates of (for instance) tea consumers among patients admitted to a hospital with some disease, with rates of tea consumers in general population in the same country?...

11 March 2020 5,560 3 View

Total of polyphenols in tea extracts conc mg/l to mg/g dry tea?

04 March 2020 3,649 7 View