Multiphysics simulation of low temperature plasma, what is the best approach?

Any thoughts on the best approach or framework to adopt here? also what is the best computational environment to use for this modelling task, ANSYS or COMSOL?

17 December 2019 3,058 3 View

Are there any open source Monte Carlo ray tracing software available for download?

Using ray tracing software to model a luminescent solar concentrator.

19 February 2019 6,861 1 View

How do I control for confounders in Minitab 17?

Dear all, I am currently investigating the effect of several predictor variables (continuous and categorical) and how they impact the response variable (continuous). The thing is, I am confused as...

07 April 2017 5,538 2 View

What post hoc test(s) are available for Mood Median test?

Dear all, I conducted a Mood Median test and determined a statistically significant difference between the medians of the 3 groups of data being analysed. However, I am wondering what post hoc...

21 February 2017 5,109 13 View

Can hoescht stain rDNA in nucleolus?

Hi, I am wondering if hoescht or dapi can stain rDNA in nucleolous. Normally they just gives a hole in the nucleus indicating nucleolus. Or if any one has a good way to visualise nucleolus...

14 November 2016 7,929 3 View

Other than Stepwise Analysis, alternatives to find significant predictors?

28 July 2016 2,278 3 View

Is a species niche the habitat that it does or could potentially inhabit, or is it a model describing that set of habitats?

03 December 2014 4,341 8 View

Zotero Vs EndNote ??

What are folks mainly using these days?

01 January 1970 3,510 12 View

Can i do harmonic response with these information in Ansys?

Hi guys, Question : can i obtain information from this picture to do harmonic response in Ansys ? if not what are the lack information ? sorry cause i got this from my researcher and i'm new to...

01 January 1970 2,747 1 View

Literature on degradation of permanent magnetic materials (e.g. neodymium)

Any thoughts and recommendations on good articles, textbooks and resources on the topic of permanent magnet materials (e.g. neodymium) in terms of phenomena associated with their ageing and...

01 January 1970 7,643 4 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View