Yes. You can use cold treatment instead of colchicine to accumulate metaphase cells. Cold treatment reduces the tendency of dividing cells to relapse back to interphase. Colchicine is a chemical treatment that poisons the spindle fibres' functionality in a way that paris of chromatids fail to move into sister cells. This results in chromosome doubling without subsequent karyokinesis. What follows is failure als of cytokinesis. Whereas cold treatment inhibits relaps or reversal of mitotic progress, thereby enhancing the percentage of metaphase cells, colchicine treatment increases the number of metaphase cells by allowing mitotic process to get to metaphaseand not beyond it thereby increasing thenumber of metaphase chromosomes. For some species one of thetwo methods can work either similarly or better than the other while the two approaches of accumulating metaphase chromosomes can be combined to acievebetter results.