Can I use a zebra fish model to find out the brain antioxidant activity of aqueous extract of some fruit seed?

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Can we use multiple linear regression with a Mediating variable for the following variables and scale points?

Two independent variables 1. Attachment style 1 to 5 likert scale Strongly disagree to Strongly agree 2. Family functioning 1 to 4 likert scale Strongly agree to strongly disagree 3. Childhood...

10 February 2024 6,384 2 View

Can anyone suggest a modified method for RNA extraction in samples having concentration of polysaccharide like pectin and xyloglucan ?

while using the TRIZOL RNA extraction method. In the precipitation step using isopropanol, polysaccharides like pectin and xyloglucan also get precipitated along with RNA, hindering the following...

30 January 2024 1,390 0 View

Is clone of Theoretical Biology Forum Journal ?

1. http://www.libraweb.net/riviste.php?chiave=114 2. http://tbf.peerjournals.net/ Which one is original

26 December 2023 2,961 1 View

How to prepare sample for low expressed secretary protein of Pichia pastoris pPCZaA, X-33 strain?

Proteins expressed in pPcZaA for protein purification using his trap column in FPLC? how to prepare a sample for that. We have lyophilized the supernatant at 24 hr, 48 hr and run SDS.

25 December 2023 9,012 0 View

How to submit reserach paper in journal?

please define steps how to upload research paper

27 November 2023 6,287 2 View

Dwell fatigue simulations using DAMASK 3.0 ?

Hi everyone, We use DAMASK 3.0. We want to do dwell fatigue simulations. Can we use phenomenological power law for dwell fatigue/creep simulations? Which Model is best for dwell fatigue/creep...

17 October 2023 3,632 0 View

In LAMP reaction I am detecting the band in NTC (control), please suggest how I resolve the issue?

I am trying to validate the detection of plant fungal pathogen through LAMP reaction. But I have an issue with the LAMP reaction. I am detecting the band in NTC (Control) also. To rectify the...

10 October 2023 1,525 2 View

Is Arrhenius plot valid for exothermic reactions?

In exothermic reactions, the rate constant decreases on increasing the temperature. When we plot the graph between ln k and 1/T, a positive slope is obtained which gives negative activation...

21 August 2023 372 1 View

How can the Thermogravimetric analysis (TGA) be used to compare similar products?

19 August 2023 3,742 1 View

Could anyone tell how can the TGA (Thermogravimetric analysis) be used to reverse engineer a product?

19 August 2023 9,667 3 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...

05 August 2024 454 3 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

How can major oxides determined by ICP-MS/ES be normalized to 100% volatile free? How does this connect with LOI?

I am analyzing cherts from different sources found within one specific geologic formation using ICP-MS/ES. My committee was asking this question about my analysis. I was not sure how to answer this

05 August 2024 7,902 3 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

How to quantify polystyrene microplastic (8 micron) bioaccumulation in fish tissue?

"I have treated adult zebrafish with 8-micron polystyrene microplastic and want to study the bioaccumulation in different organs. Can this be done using hydrogen peroxide digestion followed by...

05 August 2024 853 3 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

How to report results of Generalised Linear Mixed Models in a journal article?

Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...

04 August 2024 4,130 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View