10x RBC Lysis Buffer
89.9 g NH4Cl 10.0 g KHCO3 (out-of-stock) 2.0 ml 0.5 M EDTA
Dissolve the above in approximately 800 ml ddH2O and adjust pH to 7.3. QS to 1 liter and mix thoroughly. This solution is stable for 6 months at 2 – 8° C in a tightly closed bottle.