Hello everyone,
How can I prepare a 0.1 M Citrate-Phosphate Buffer for pHs 4.0, 6.0 and 8.0, using the solids Citric Acid (C6H8O7 MW: 192,12 g/mol) and Disodium Hydrogen Phosphate (NA2HPO4 MW: 141,96 g/mol)? I tried to do some calculations but it didn't work.
Thank you!
Hi Giovana,
This link will help you to prepare your buffer.
https://www.cusabio.com/m-296.html#a15
Dear Duried, thank you, however this website uses different molarity and I could not do the calculations for the molarity I want. I really like to know how to make the calculations for this buffer
I'm not sure if rhe answer is Henderdon Hasselbach.
Hi! I need the same information, so If you finally got how to calculate, could you please tell it me? Thanks Giovana Colucci
About predicting the pH of citric acid ― sodium citrate solutions and buffers for approx. pH < 4.0 ― cf. my post at:
https://www.researchgate.net/post/How-to-prepare-a-citric-acid-solution-of-pH-25-and-3-from-100-citric-acid-powder
About predicting the pH of citric acid ― sodium citrate solutions and buffers for approx. 4.0 < pH < 5.5 ― cf. my posts at: https://www.researchgate.net/post/Why-the-pH-of-Citrate-buffer-increases-when-diluted-with-water
About predicting the pH of citric acid ― sodium citrate solutions and buffers for approx. 5.5 < pH < 8.0 ― cf. my posts at: https://www.researchgate.net/post/Why-the-pH-of-Citrate-buffer-increases-when-diluted-with-water
About predicting the pH of citric acid ― sodium citrate solutions and buffers for approx. pH > 8.0 ― and for approx. pH > 9.0 ― cf. my posts at:
https://www.researchgate.net/post/Why-the-pH-of-Citrate-buffer-increases-when-diluted-with-water
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