This may be disputed; I prefer glass vials for keeping protein solutions, especially at low protein concentrations. Once I noticed slight adsorption of myoglobin from its 0.1 mg/mL solution on plastic Erlenmeyer flask.

Adsorption of BSA on surfaces is low, but if you want to avoid it you can get your surfaces siliconized.

I am using .84mg/80ml. A.Ivanov how do you notice adsorption? I'll keep my samples ,CoCrMo, immersed on the solution that has BSA for 20 days.

Yann for siliconized thank you for your suggestion

Hi Rana and Yann-Vai. I noticed adsorption by measuring myoglobin absorbance at 410 nm, it was about 2 - 3 % decrease after some hours but still interfering with the protein adsorption on carbon, which I studied. Yes, some glassware is siliconized. It would be good to know, however, which kind of organosilanes were used by the manufacturers and for which purpose. I would suppose that hydrophobic silane coatings may even induce protein adsorption.

Hi A.Ivanov, so what should I do to avoid protein adsorption on glass? should I use other type of container?

Thank you so much.

Hi Rana, as I wrote before, glass vessels seem to me more inert compared to plastics. I do not think you may have significant decrease in BSA concenration due to adsorption on glass walls, especially if you have some ionic strength. If you are interested in those slight possible concentration changes, make control experiment with Bradford reagent (or just measure protein absorbance at 210 nm) immediately after protein contact with glass and after 20 days. By the way, if you keep BSA in solution for 20 days, some preservative like 0.02% sodium azide will be helpful.

Alexander

Thank you so much A. Inanov. that's really a great help. I am wondering why should I use the preservative? because I want to see how BSA adsorbs normally with CoCrMo ?

The preservative will prevent bacterial attack, which is possible in protein solution during long storage. Typically, protein solutions start smelling at RT in some days.