Unfortunately, I had to stope expression process for about 5 months and after this delay time I had to again to do some change in expression conditions I mean against before I used 25◦C to participate bacterial suspension. But after purification step, there was no band in SDS PAGE. I don’t know what is the wrong? Is this possible that this change in centrifugation temperature caused I lost my protein? My protein is HIV-1 Tat and Expression temperature is 37◦C for 4 hours and I use BL21DE3 and PET28(a).
Aamal Ghazi Mahdi Al-Saadi
https://link.springer.com/chapter/10.1007/978-981-287-817-5_39
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