is there any relationship between position of a residue in secondary structure and solvent accessibility ?
I have a cysteine-rich domain in my recombinant protein. results of SDS-page in reducing and non-reducing condition improved the presence of oligomers caused by disulfide bond between monomer...
06 July 2017 3,820 4 View
I want to study the structure of my protein using intrinsic fluorescence spectroscopy. some times I see the emission peak at 380nm? is my protein aggregated? or there is another reason for...
05 June 2017 1,764 4 View
what happens when the intensity of fluorescence spectra enhanced in high concentration of GnHCl (3M)?
03 April 2017 9,470 3 View
as you know chitosan is soluble in acidic pH but I want to use it in vaccine formulation, so I need to adjust it in physiologic pH but when I tried for this , it participated ! what can I do?
03 April 2017 10,016 4 View
Unfortunately, I had to stope expression process for about 5 months and after this delay time I had to again to do some change in expression conditions I mean against before I used 25◦C to...
03 April 2017 7,279 1 View
03 April 2017 5,142 4 View
I need to do some experiment with it in liquid form.
07 August 2024 613 3 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Because I have realized that the world tends more and more to do open and free science and there is a trend more and more to choose free databases, free tools and open access platforms.
01 August 2024 10,046 1 View
Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?
01 August 2024 7,504 2 View
HI there, I've came across several articles discuss about random audit an Non random to tax evasion or compliance. Most of the articles is relating about effect of audit (random or non random)...
31 July 2024 5,309 7 View
I am currently conducting a mendelian randomization study, and I was attempting to use PhenoScanner to look for potential confounders associated with the selected SNPs (any SNPs significantly...
23 July 2024 5,703 0 View
Why methanol and sulphuric acid, used in the analysis of polyhydroxyalkanoates (PHA) methyl esters by GC-MS? Additionally, why do we typically use non-polar solvents in GC-MS?
22 July 2024 1,210 2 View
Is it the best optimization ratio based on experiments?
22 July 2024 8,170 0 View
If you don't understand my question let me know
21 July 2024 563 1 View
I used several solvents and measured the absorbency and determined the total phenolic content, but the results were similar after dropping them on the titration curve; What is the reason, please help
17 July 2024 5,151 5 View