Can your Imagination will Imagine? What you think about real future World in various sectors because of digital & wireless innovations? What are SWOT?

Can your Imagination will Imagine? What you think about the real future World in various sectors because of digital and wireless innovations? How it will change the World? What will be the...

21 December 2020 3,506 7 View

Can Antibody-NHS activated resin complex withstand 1% SDS solution?

I have generated a customized NHS-activated column which is coupled to a particular antibody. As per the manufacturer's protocol (Pierce, Thermo Scientific) I was isolating native protein in...

04 October 2020 8,236 3 View

Why second stage juveniles are used for DNA extraction from nematode for identification purpose? Can I use female or male for the same purpose?

I am working on rice root knot nematode. For identification purpose mostly second stage juveniles are used. I wonder why females or males are not used commonly for the same purpose? I am using...

26 March 2020 7,419 3 View

What is your opinion about patents filed by Academic Institutes/ Universities ?

What is your opinion about patents filed by Academic Institutes/ Universities ?

04 January 2020 9,884 3 View

Glucose-6-phosphate and glucose-2-phosphate metabolism in bacteria when used as an exogenous carbon source?

Can expert inform the metabolic pathway when we feed exogeneous glucose-6-phosphate as a carbon source to bacteria (aerobic cultivation). Also requesting the metabolic pathway when exogenously...

18 August 2019 4,581 4 View

What you do for increasing student's interest and attendance in class?

What you do for increasing student's interest and attendance in class?

15 August 2019 133 10 View

How to increase the utilization of renewable energy? What are the barriers for utilization of renewable energy sources?

There are many possible applications and reasons of utilization of renewable energy. Please share your opinion where and how renewable energy utilization can be increased? Also, share your...

03 August 2019 8,105 15 View

Which energy source you prefer for cooking, considering sustainable future? And why?

Which energy source you prefer for cooking, considering sustainable future? And why? Electricity, Solar Energy, Wind Energy, Biomass Energy, Gas/Propane/Heating Oil, kerosene, methanol, ethanol...

31 July 2019 5,000 32 View

How to create research interest in engineering graduate students?

How to create research interest in engineering graduate students?

23 December 2018 6,281 3 View

Is there any microorganism who uses carbon monoxide for their metabolic activities ??

I want to work on that microbe but I didn't get it

21 December 2018 5,297 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View