Is it acceptable to cut the western blot membrane into multiple pieces to simultaneously probe for different proteins?

Hello, Few publishers usually ask to provide the full length image of the western blot membrane (in supplementary). If this is that case, how should someone report the results, when they cut their...

11 December 2019 2,448 3 View

How do we decide the time point for Proteomics after treatment?

I am interested in looking at protein profile after the treatment on the breast cancer cells, using Proteomics. However, I am not quite sure about the time point at which it will be the best to do...

07 August 2018 7,249 3 View

Why does not CyclinD1 expression change in MDA-MB-231 cells, even when I see the cells trapped are in G0/G1 phase of the cell cycle ?

After my treatment, I can see that most of the cells are trapped in G0/G1 phase of cell cycle. But when I probe my cell lysates against Antibody of CyclinD1 in westernblots, I do not see a reduced...

10 November 2017 9,269 1 View

Why do human breast cancer (MDA-MB-231) cells form cluster after 24 hour of treatment with the Drug?

I treated MDA-MB-231 cells with my drug in suspension. I plated cells into 96 well plate after the treatment and incubated for 24 hours. I observed my samples into the microscope, and found that...

31 December 2016 7,908 9 View

Why do MDA-MB-231 cells express less protein in BCA Assay before westernblotting?

I treated MDA-MB-231 cells in suspension with electroporation and drug (~3 million cells per treatment). I prepared cell lysate and performed BCA protein assay to know that I would need 200 ul of...

31 December 2016 9,126 3 View

Is it possible to capture 4 Ghz signals with a DSO of 3Ghz bandwidth?

06 July 2014 650 4 View

Why doesn't my log file (md.log) show the same parameters value as in parameter file (md.mdp)for generating alternating electric field in GROMACS?

01 January 1970 5,113 0 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View