Lakshya Mittal

8 Questions 6 Answers 0 Followers

Questions related from Lakshya Mittal

Is it acceptable to cut the western blot membrane into multiple pieces to simultaneously probe for different proteins?

Hello, Few publishers usually ask to provide the full length image of the western blot membrane (in supplementary). If this is that case, how should someone report the results, when they cut their...

12 December 2019 2,601 3 View

How do we decide the time point for Proteomics after treatment?

I am interested in looking at protein profile after the treatment on the breast cancer cells, using Proteomics. However, I am not quite sure about the time point at which it will be the best to do...

08 August 2018 7,346 3 View

Why does not CyclinD1 expression change in MDA-MB-231 cells, even when I see the cells trapped are in G0/G1 phase of the cell cycle ?

After my treatment, I can see that most of the cells are trapped in G0/G1 phase of cell cycle. But when I probe my cell lysates against Antibody of CyclinD1 in westernblots, I do not see a reduced...

11 November 2017 9,329 1 View

Why do MDA-MB-231 cells express less protein in BCA Assay before westernblotting?

I treated MDA-MB-231 cells in suspension with electroporation and drug (~3 million cells per treatment). I prepared cell lysate and performed BCA protein assay to know that I would need 200 ul of...

01 January 2017 9,192 3 View

Can I study NFkB pathway in breast cancer cell line MDA-MB231 without stimulation by TNF-a ?

I am working on a drug that is supposed to block NF-kB, so I want to study the effect of the drug in breast cancer cell line MDA-MB-231. NFkB is already active in MDAMB231 cell lines, should I...

01 January 2017 8,280 2 View

Why do human breast cancer (MDA-MB-231) cells form cluster after 24 hour of treatment with the Drug?

I treated MDA-MB-231 cells with my drug in suspension. I plated cells into 96 well plate after the treatment and incubated for 24 hours. I observed my samples into the microscope, and found that...

01 January 2017 7,977 9 View

Is it possible to capture 4 Ghz signals with a DSO of 3Ghz bandwidth?

I want to capture one signal containing frequency in the band of 1-5 Ghz but i have a Digital storage oscilloscope of 3 Ghz bandwidth only. Is there any chance to capture the signal above 3 Ghz...

07 July 2014 717 4 View

Why doesn't my log file (md.log) show the same parameters value as in parameter file (md.mdp)for generating alternating electric field in GROMACS?

I am trying to simulate alternating Electric Field in GROMACS. However, when I set up parameters for gaussian (E-zt) in my parameter file and do the MD production run, I get different values in...

01 January 1970 5,167 0 View