I did bovine PBMC after Ficoll centrifugation suspended in PBS at 4 degree for 5 day and after this 70% are vital (PI neg). For store at - 80 degree you had to use DMSO 10% I think
Hi! you can freeze your cells, but you need a specific protocol for this. I suggest you to take a look on this one, is very detailed and it includes a list of all the materials you need: http://www.biomedcentral.com/content/supplementary/1471-2172-6-17-s1.pdf. About storing at 4ºC, I would say it depends a lot on the kind of experiments that you want to do with them. A few hours should not be a problem, but if you are talking about days, then you would need to include good positive and negative controls, just to make sure that your cells are "working properly". I hope this helps!
In some studies in PBMC in wild animals, lymphocytes recovering decreases dramatically after 8 hours at 4º C, at least for proliferation assays.
http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2249.2010.04272.x/full
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