Is it possible to store cells stained with 7AAD for 12 hours? And do I need to fixate them after staining? Thank you.
Hi, everyone! Could anyone share their experience with cryostorage? I would like to know, how many vials of which passage do you usually freeze to maintain a cell line in culture? Both for...
07 May 2023 520 6 View
Is it possible to compare the transformation of the traditional teachers to the digital reality with immigration? Is this transformation a real immigration? What do you think?
10 April 2021 3,443 3 View
I am interested in your opinions about the future of the educational system ? Will it be digital? Will it be Hybrid ( mixed off-line and on-line)? How the whole teaching approach is going to be...
08 March 2021 1,898 60 View
The final qualification assessment for major programs is a strict regimen that involves different stages - oral and written exams, final project viva, internal and external review. How are ICT...
16 February 2021 2,185 8 View
English Business Communication and Soft skills became the necessary basis for doing business in the global digital era. Wherever business communication fails, the business loses money and becomes...
14 January 2021 6,351 6 View
I need to measure NO production by in vitro generated mouse MDSCs. Since I cannot store diluted Griess reagents for a long time, I want to collect supernatants and then analyze them all together....
31 October 2018 5,765 1 View
FM 4.64 worked well only in Arabidopsis roots.
01 March 2018 3,295 3 View
I am looking for a good marker of NFkB activation in order to analyze it in lymphocytes obtained from inflamed mice by flow cytometry. Would antibodies to phophorylated IkB alpha, such as...
26 February 2018 1,352 3 View
We would like to submit the application for the Visegrad fund and search partners from Hungary, Slovakia and Poland. The project is associated with a removal of pharmaceuticals on the constructed...
26 September 2017 5,589 3 View
substances, concentration, physical methods
20 August 2017 9,643 2 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
09 August 2024 9,894 2 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...
07 August 2024 9,563 0 View