How can we assess applied digital skills and soft skills through online examinations during COVID-19 semester?

The final qualification assessment for major programs is a strict regimen that involves different stages - oral and written exams, final project viva, internal and external review. How are ICT...

16 February 2021 1,762 8 View

What's the difference between the role-plays and real-world cases in teaching Business English?

English Business Communication and Soft skills became the necessary basis for doing business in the global digital era. Wherever business communication fails, the business loses money and becomes...

14 January 2021 6,046 6 View

Can anyone suggest a good marker of NFkB activation for the analysis by flow cytomtery?

I am looking for a good marker of NFkB activation in order to analyze it in lymphocytes obtained from inflamed mice by flow cytometry. Would antibodies to phophorylated IkB alpha, such as...

26 February 2018 1,295 3 View

Has anyone worked with HLA-G1 transcripts? Could you please say the primers?

Has anyone worked with HLA-G1 transcripts? Could you please say the primers? HLA-G1 isoform are detected with exon3 and 3'-UT primers, but we can't find the 3'-UT primer. Thank you in advance

18 January 2015 5,405 1 View

What is a good nitrate meter for the in situ measurement of natural waters?

Thank you in advance for advantages and disadvantages!

27 June 2014 6,037 7 View

When you use tandem dyes (PerCP-Cy5.5 or APC-Cy7 or others) what buffer solution do you keep your cells in if you can't analyze them immediately?

Please could anyone say when you use tandem dyes (PerCP-Cy5.5 or APC-Cy7 or others) what buffer solution do you keep your cells in if you can't analyze them immediately? Thanks in advance!

20 January 2014 2,072 11 View

Conference for Skills Development?

Would you recommend a conference with an academic publication on Skills Development and Assessment? I am interested in Soft Skills, Digital Skills and Communication skills, they also may refer to...

01 January 1970 8,379 5 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View