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Questions related from Yuliya Perfilyeva
I need to measure NO production by in vitro generated mouse MDSCs. Since I cannot store diluted Griess reagents for a long time, I want to collect supernatants and then analyze them all together....
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I am looking for a good marker of NFkB activation in order to analyze it in lymphocytes obtained from inflamed mice by flow cytometry. Would antibodies to phophorylated IkB alpha, such as...
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Has anyone worked with HLA-G1 transcripts? Could you please say the primers? HLA-G1 isoform are detected with exon3 and 3'-UT primers, but we can't find the 3'-UT primer. Thank you in advance
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Please could anyone say when you use tandem dyes (PerCP-Cy5.5 or APC-Cy7 or others) what buffer solution do you keep your cells in if you can't analyze them immediately? Thanks in advance!
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