Yuliya Perfilyeva

5 Questions 4 Answers 0 Followers

Questions related from Yuliya Perfilyeva

Is it possible to freeze cell culture supernatants and store them before analyzing NO production by Griess reaction?

I need to measure NO production by in vitro generated mouse MDSCs. Since I cannot store diluted Griess reagents for a long time, I want to collect supernatants and then analyze them all together....

01 November 2018 5,798 1 View

Can anyone suggest a good marker of NFkB activation for the analysis by flow cytomtery?

I am looking for a good marker of NFkB activation in order to analyze it in lymphocytes obtained from inflamed mice by flow cytometry. Would antibodies to phophorylated IkB alpha, such as...

27 February 2018 1,389 3 View

Has anyone worked with HLA-G1 transcripts? Could you please say the primers?

Has anyone worked with HLA-G1 transcripts? Could you please say the primers? HLA-G1 isoform are detected with exon3 and 3'-UT primers, but we can't find the 3'-UT primer. Thank you in advance

19 January 2015 5,491 1 View

Can I store cells stained with 7AAD overnight?

Is it possible to store cells stained with 7AAD for 12 hours? And do I need to fixate them after staining? Thank you.

08 January 2015 2,377 0 View

When you use tandem dyes (PerCP-Cy5.5 or APC-Cy7 or others) what buffer solution do you keep your cells in if you can't analyze them immediately?

Please could anyone say when you use tandem dyes (PerCP-Cy5.5 or APC-Cy7 or others) what buffer solution do you keep your cells in if you can't analyze them immediately? Thanks in advance!

21 January 2014 2,137 11 View