Is there any relevant literature on the correlation/relationship between sustainability on a national and company level?

I am currently writing my Master thesis w ith the tilte: Does sustainability correlate on the national and the company level? Empirical evidence from selected country cases. I struggle to find...

17 June 2020 4,316 4 View

Is mediation analysis adequate to answer whether subjects that respond to X strongly are also those that perceive it in a certain way?

Consider an experiment where an intervention X was manipulated and the behavioral outcome Y observed. After observing the outcome, a post-experimental questionnaire asked respondents about their...

26 May 2019 1,439 2 View

Tunable protein expression in E. coli?

12 March 2019 316 3 View

On which day of the week was Record Retailer published during the 1960s?

And what days prior to that day of the week did the charts published represent?

23 January 2019 6,604 1 View

How can I set a cell zone in OpenFoam to Overpressure?

Hey fellows! I would like to simulate a pressurised outlet stream of air. In the simulation I'll have some kind of a container filled with an overpressure of 2 bar air and I would like to...

23 October 2018 612 2 View

Where can i find a Matlab code using a Centroidal Voronoi Tessellation on Ellipsoids?

I found this algorithm which works quite well for spheres but i cant seem to rewrite it to work for ellipsoids:...

19 October 2018 3,804 2 View

How to test for causal mediation with instrument for mediator?

I've got a question regarding the intersection of mediation and instrumental variables. I want to test if a randomized treatment variable (T) has a direct effect on outcome (Y), as well as an...

28 May 2018 1,672 6 View

Type I error control dependent on experimental design?

If I want to test two hypothesis, i.e. whether Treatment 1 (T1) affects outcome O, and whether Treatment (T2) affects outcome O, relative to a control group (C), does it make a difference with...

13 June 2017 7,276 3 View

Regressing change score conditionally censored on pre-test outcome

I conducted an experiment where subjects made two decisions. the first (Pre) decision was unaffected by any treatment, whereas subjects made their second decision (Post) after being randomly...

23 May 2017 8,925 3 View

Is there a difference between ANOVA analysis of change scores and repeated-measures ANOVA?

I measure a continuous variable prior and after treatment allocation in a 3x4 factorial experimental design . The pre-treatment measurements are all unaffected by the treatment. There is no...

21 November 2016 2,878 4 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View