How do I calculate the correct primer concentration or molarity?

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Sabine Strehl

Maybe this website will help you to solve your question:

https://www.physiologyweb.com/calculators/dilution_calculator_molarity_percent.html

Aissa Saidi

The rule is that: 1µM = 1pmol/µl,

in your case, if you take 0.4 µL of 10µM (10pmol/µl) concentrated primer, the final concentration will be 0.16 µM (160nM) in a final reaction volume of 25µl.