5 Questions 19 Answers 0 Followers
Questions related from Andrea Guedez
I am doing flow cytometry for cell cycle with E. coli after induction of proteins that cause cell death. The procedure I followed consists in growing cells to an OD of around 0.2 take a pre...
20 May 2025 5,840 1 View
I am trying to determine MICs but I need to express proteins in order to get the antibiotic resistance I am looking for. I have used enriched minimal media, but we are exploring another conditions...
07 September 2023 1,794 2 View
Hello, I submitted two samples for whole plasmid sequencing. I usually get a fasta. fastaq, the histogram and the consensus file. This time they only attached the fastaq and the histogram file....
24 May 2023 8,399 3 View
I am expressing CAT-intein fusions and somehow the c-term fragment is experiencing degradation. I have seen few papers that show the main product and some degradation products but don't give much...
25 April 2023 1,381 1 View
I cut the bands of interest after loading the proteins to the gel but I would like to keep them for a further MS analysis. Should I leave them with water? acetic acid solution? some specific...
10 July 2019 2,803 6 View