Andrea Guedez

5 Questions 20 Answers 0 Followers

Questions related from Andrea Guedez

Can you offer some guidance with flow cytometry cell cycle for E. coli?

I am doing flow cytometry for cell cycle with E. coli after induction of proteins that cause cell death. The procedure I followed consists in growing cells to an OD of around 0.2 take a pre...

20 May 2025 5,851 1 View

Is the Mueller Hinton Broth good for protein expression?

I am trying to determine MICs but I need to express proteins in order to get the antibiotic resistance I am looking for. I have used enriched minimal media, but we are exploring another conditions...

07 September 2023 1,799 2 View

If I only get a fastaq file from my whole plasmid sequencing order does it mean that it failed?

Hello, I submitted two samples for whole plasmid sequencing. I usually get a fasta. fastaq, the histogram and the consensus file. This time they only attached the fastaq and the histogram file....

24 May 2023 8,406 3 View

Has anyone had issues expressing CAT or CAT-intein fusions?

I am expressing CAT-intein fusions and somehow the c-term fragment is experiencing degradation. I have seen few papers that show the main product and some degradation products but don't give much...

25 April 2023 1,387 1 View

What is the best way to store SDS PAGE bands?

I cut the bands of interest after loading the proteins to the gel but I would like to keep them for a further MS analysis. Should I leave them with water? acetic acid solution? some specific...

10 July 2019 2,811 6 View