I have extracted the DNA for Acinetobacter baumannii using extraction kit.I want to make sure if the DNA is successfully extracted before going into PCR. If I can, what percent of agarose should i use?
Ganapati Bhat
For genomic DNA I usually use 0.8% agarose gel. You can see DNA band near the well.
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Mahesh Karale
you use 0.8% agarose gel or decrese the concentration of agarose i.e. 0.7 of 0.6% to see the bands of your isolated DNA.
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Paul Rutland
do not bother . Just run the pcr.even small degraded genomic dna will amplify fine ( sometimes even better than large intact dna ( small melts better) and anyway you can often pcr from invisibly small amounts of dna. Borrow a good set of a colleagues primer if you are unsure of whether your primers will work
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