Johan Nathan

3 Questions 3 Answers 0 Followers

Questions related from Johan Nathan

How to troubleshoot failed salt test in passive conjugation of AuNP and antigen?

Dear colleagues and experts, We were trying to conjugate an antigen with 48kDa with 40nm AuNPs. We tried using 20ug of antigen (diluted in 20mM PB, 150mM NaCl,1‰P300 buffer) + 250ul of AuNPs OD20...

12 May 2023 3,513 3 View

Where to purchase certain strain of bacteria?

Hello, I am wondering if I can purchase a certain strain of bacteria (P. aeruginosa VA-182). I am going to use it as positive control for PCR.

01 August 2016 1,437 3 View

Can I run 3000kbp bacterial genomic DNA in electrophoresis just to know it the DNA is sucessfully extracted?

I have extracted the DNA for Acinetobacter baumannii using extraction kit.I want to make sure if the DNA is successfully extracted before going into PCR. If I can, what percent of agarose should i...

28 July 2016 5,297 4 View