I occasionally have to measure protein concentrations. For this I use the BCA Protein Assay Kit by ThermoFisher. I never use all of the standards. Is there a way to store them, so I can reuse them a week or so later?
Hi, I need to measure the real-time (or temporal intervals) thickness variation of a transparent material from dry to wet/humid conditions, however, I cannot use the following techniques: -...
08 July 2024 4,799 4 View
Dear fellow researchers, We are currently operating a Philips XL30 Scanning Electron Microscope (SEM) equipped with a tungsten filament and have encountered a significant issue. Recently, our...
28 May 2024 232 2 View
28 May 2024 9,415 2 View
I am currently using folded paper lichen/bryophyte packets, but I suspect a more rigid container might be better? Any specific suggestions? And if so, how do you attach a permament label to the...
14 February 2024 5,559 1 View
Dear Colleagues, In recent weeks, I have suffered from an issue with the SmartLab SE Diffractometer (Rigaku). Last week, I started the equipment as usual and an error appeared; please see the...
09 January 2024 4,984 10 View
For phenotyping disease resistance/susceptibility in a plant, we have digitally scanned the leaf area of many leaves over a time period after inoculation (controlled environment). Would using a...
04 January 2024 10,031 2 View
I have a question regarding the expected light power at the output port of an integrating sphere. What light power can be expected at the output port of an integrating sphere when a fiber laser...
23 April 2023 1,873 1 View
I am doing a trial for my bachelorthesis and examine the roots of the experimental plants (betula grossa) with the intersection gridline method - we used a commercial mycorrhizal mix (with some...
09 September 2022 2,413 0 View
I am offering a special Boron Carbide mortar and pestle which I have used over the last two decades for crush-leach-Rb-Sr isotopic analysis uf sphalerite/quartz and its fluid inclusions. The...
29 April 2022 3,491 1 View
My samples are in a lysis buffer that has proteinase inhibitors added to it. Do I have to add these inhibitors to the buffer in which I dilute the standards? Because Im not sure whether it...
30 March 2022 6,719 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...
31 July 2024 193 4 View
I did an MTT assay with 2 drugs (Piroxicam and Sulfameter) when I got my results for the 12,24,48,72 hours of incubation with my drug at the following concentrations [ug/ml]...
26 July 2024 7,511 3 View
Hello, I want to ask regarding exosome protein quantification method. Is it necessary to lyse exosomes before measuring their protein contents using BCA assay kit? Or can I just straight away...
23 July 2024 4,603 4 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
Despite not having cells in the media, I am getting purple color. I have tried many troubleshooting methods, varying media types, and even different MTTs from different companies to figure out the...
21 July 2024 9,914 1 View