Botho Schneider

4 Questions 4 Answers 0 Followers

Questions related from Botho Schneider

BCA-Assay. Do I have to add Proteinase Inhibitors to the buffer in which I dilute my albumin standards to get an accurate result?

My samples are in a lysis buffer that has proteinase inhibitors added to it. Do I have to add these inhibitors to the buffer in which I dilute the standards? Because Im not sure whether it...

31 March 2022 6,737 4 View

Can I reuse protein standards from the BCA Protein Assay Kit?

I occasionally have to measure protein concentrations. For this I use the BCA Protein Assay Kit by ThermoFisher. I never use all of the standards. Is there a way to store them, so I can reuse them...

27 October 2021 4,340 0 View

Does someone have experience with reusing coomassie dye?

I stained a western blot gel with coomassie dye that has been used multiple times before. The staining worked, but it was very light. First we thought that the protein concentration in the sample...

05 July 2021 5,448 7 View

Protein solubilization for western blot after lysation in Co-IP lysis buffer. How to add the denaturing detergent?

I've lysed some cells for Co-IP. For this purpose the lysis buffer is without denaturing detergents. For western blot I now need to add SDS or another appropiate detergent. Can I just add laemmli...

01 July 2021 3,607 3 View