Hi,

I would be grateful for your opinion.

I performed a DGE analysis and received a list of differentially expressed genes.

Next, we focus on specific list of pre-defined genes for further analysis, since their biological functions are relevant for our scientific question (and all other differentially expressed genes will not be further included).

Since the list of genes-of-interest was selected by previous research I thought to run a functional enrichment analysis on those pre-defined genes to reassure that they are truly represent the biological functions we assume they are.

That is different from functional enrichment analyses I performed in the past, which were on all the differentially expressed genes.

Do you think our approach is reasonable?

thanks!

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